Savanna Journal of Basic and Applied Sciences 3(1):81-85
Chromatographic and Antidermatophytic Evaluations of Essential Oil Compounds from Citrus sinensis Fruit Peel
Ungo-kore H.Y., Ehinmidu, J.O., Onaolapo, J.A. and Olonitola, O.S.
ABSTRACT
Essential oils (EOs) are complex natural mixture of chemical constituents, having a major component at a relatively high
concentration while the minor ones are present in trace amounts. Hence, the need for a simple and efficient isolation technique
required to obtain compound that are bioactive antidermatophytic. Therefore, the study aim to isolate bioactive molecules of EO
from fruit peels of Nigerian Citrus sinensis (L.) Osbeck with potential antidermatophytic activity against multidrug-resistant tinea
capitis dermatophyte. The EO was reaped by hydrodistillation and the bioactive components were examined by thin layer
chromatography coupled with direct bioautography assay method. The latter is an assertive technique that targets the isolation of
only the active component(s) in a bioassay-guided fashion. The antidermatophytic components were identified with GC-MS as 4-
Heptyn-2-ol and Pentane-2, 4- dimethyl. These components (mixture) recorded, are a first-hand report—as present in Citrus
sinensis (L.) Osbeck fruit peel EO. Our results support a diagnostic method in the isolation of antidermatophytic components from
essential oil with an additional confirmation step.
