Savanna Journal of Basic and Applied Sciences 1(2):231-234
Characterization of Bacteria from the Gut of Sitophilus zeamais (Maize Weevil)
Danjumma B.J., Shamtau D. Yusuf M., and Peni D. N.

Sitophilus zeamais is ranked among insects causing the greatest damage in stored to maize grains in most parts of the tropical and sub-tropical countries. The activities of the pests are so destructive that damage ranging from 20 to 90% has been recorded on the untreated maize. The current work focus on bacteriological isolation and identification of bacteria found in the gut of S. zeamias. Hetetrophic plate counts was carried out using pour plate method, morphological features of the bacterial by colonies appearance on the plate media and identification by microscope and biochemical test. The results showed that nutrient agar plate had Total heterotrophic bacteria count of 33x10 7 cfu/ml, Total Enterobacter count in MaCconkey agar was 17x10 7 cfu/ml and the Total coliform count in Eosin Methylene Blue agar was 32x10 -7 cfu/ml. Five (5) different bacteria from the gut adults S. zeamais were isolated. The members of the genera isolated were Bacillaceae and Enterobacteriaceae families were isolated from S. zeamais. The results of biochemical tests conducted revealed the present of E. coli, Enterobacter sp, Acetobacter spp. Bacillus spp. and Gluconobacter spp. This study confirms that the gut of S. zeamais harboured. The bacteria isolated produces enzymes capable of degrades chitin, a structural polysaccharide in maize grains. Thus, the S. zeamais and the microorganisms inhabiting the gut share a highly symbiotic relationship.