Fungi associated with the spoilage of bread in Birnin Kebbi were investigated and identified. Three different bread samples were collected in Birnin Kebbi metropolis namely; Kampala, Karama and Adalci bread. Each bread was kept sealed and exposed for 7days at different locations under ambient environmental conditions of Relative humidity and room temperature and observed daily for spoilage. The organisms obtained to be associated with the spoilage of bread were Aspergillus niger, Mucor racemous, Aspergillus fumigates, Aspergillus flavus, A. terries, Cephlosporium species, and Paecilomyces species. Percentage of occurrence varies from 17.30% to 8.75%. Since these fungi are known to produce aflatoxin. However, from the data and results obtained in this research, bread was shown to be seriously contaminated after 7 days and is unfit for human consumption as a result of the growth of these toxic producing different fungal species due to improper storage facilities and which would cause toxin which can cause serious health problem to human beings and even animals when consumed.