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Savanna Journal of Basic and Applied Sciences 1(1):140 - 144
Synergistic Effect of Alkaloid and Saponin of Bitter leaf (Vernonia amygdalina) Against Escherichia coli
A. B. Yusuf, S. Murtala, I. E.Obaro and A. A. Turaki

ABSTRACT
The work was aimed at studying the Synergistic effect of alkaloid and saponin compound of the Vernonia amygdalina against Escherichia coli. Ethanol and aqueous were used as solvent for crude extraction. The antibacterial activities of the alkaloid, saponin and their synergistic effect were carried out at varying concentration of 100, 50, 25, 12.5 and 6.25 mg/ml, using agar well diffusion method. The result of this study indicate that, ethanolic extract shows higher anti-bacterial activity at 100mg/ml concentration (11.29 mm) compared to aqueous extract (9.24mm) against the test bacteria. It also showed that the anti-bacterial activity of the alkaloid content of the Vernonia amygdalina at 100 mg/ml (11.01mm) was better than that of saponin (9.32mm). The result revealed that synergistic effect of alkaloid and saponin at concentration 100 mg/ml (15.41mm) was better than the individual compounds. The result demonstrates the possibility of using the alkaloid and saponin compounds synergistically in the treatment of disease associated with Escherichia coli