Nigeria has several native leafy vegetables that have been neglected. Corchorus olitorius belongs to this group. Genetic diversity was studied in five accessions of the plant using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS - PAGE). The aim was to determine relationship (differences and similarities) among them. Sixteen polypeptide bands were obtained ranging from 12.29 KDa to 170 KDa indicating variations in the banding patterns of the accessions. The unweighted pair group method with arithmetic algorithm (UPGMA) dendrogram grouped the five accessions into three clusters with four groups showing 60 per cent of the accessions in one group. The greatest similarity (96 per cent) observed was between NGB00196 and NGB00194 while the lowest similarities (32 per cent) NGB00191 and (40 per cent) NGB00187. Adopting this technology can be useful in plant variety identification and registration of new plant varieties. NGB00191 was observed to be distantly related to the other accessions hence could be combined in a breeding programme.