Dambu Masara has been consumed in different parts of the world especially North western Nigeria. Its use is however limited to spoilage by microorganisms. This study was carried out to isolate and identify fungi species responsible for the spoilage of Dambu Masara in Birnin Kebbi. Two areas were selected for samples collection namely; Badariya and Go slow area all in Birnin Kebbi metropolis. Forty (40) samples of Dambu Masara were obtained in each sample site. A total of eighty (80) samples of dambu masara were collected all in all and analyzed. Using culture growth medium (Sabroud potato dextrose agar) and poured plate method fungi were analyzed. A total of five (5) fungal species were isolated and identified from the both samples as; Aspergillus niger, Penicillium spp., Rhizopus stolonifer, Aspergillus fumigatus and Mucor spp. The highest percentage of occurrences were obtained in A. niger (58.5%) while lowest were recorded A. fumigatus (2.1%). Base on locations, Go slow has the highest mold load of 83% while Badariya had the lowest 60%. Moulds are known to cause spoilage of Dambu products for the results obtained. Therefore when they are available in significant number, they lead to huge economic loss in addition to the pathogenic species causing health dangers. The implication of this study is that the process and quality of locally produced Dambu needs to be critically monitored to avoid transmission of infection to consumers.